Figure 5: BAL cell expression of Interferon-γ expression is not enhanced by ESAT-6. IFN-γ mRNA ascertained by qPCR was comparably and significantly (p ≤ 0.05) elevated in MWCNT and MWCNT + ESAT-6 compared to sham controls. IFN-γ was undetectable in BAL cells from mice receiving ESAT-6 alone.