Gene Primer Sequence (5’-3’) Temp (oC) ALF2 Forward GGAAAGTACTCCGTGACCCCAAAGC 59 ALF2 Reverse TCCCGAGGAGTTCAGTTCAGCAGTT ALF4 Forward ACTAACCCTTTCGCTCCCACCCAC 59 ALF4 Reverse CCCCACCGAGAGGTAGTAAAATGCTT LGBP Forward CATGTCCAACTTCGCTTTCAGA 57.3 LGBP Reverse ATCACCGCGTGGCATCTT ProPo Forward GCCTTGGCAACGCTTTCA 57.8 ProPo Reverse CGCGCATCAGTTCAGTTTGT SP Forward CGTCGTTAGGTTAAGTGCGTTCT 56.3 SP Reverse TTTCAGCGCATTAAGACGTGTT 18s Forward TATACGCTAGTGGAGCTGGAA 55 18s Reverse GGGGAGGTAGTGACGAAAAAT

Table 2: Specific primers used to evaluate immune status of shrimp, L. vannamei.