Figure 2: Conventional gene delivery with naked adenoviral vectors yields low efficiency in c2c12 myotubes: C2C12 myoblasts and fully differentiated myotubes were incubated for 60 minutes with a recombinant adenoviral vector, RAd-GFP, at a MOI of 60 viral particles per cell. To assess GFP expression, cell culture imaging and fluorescence quantitation were performed 48 hours after infection. A Representative microscopy images of myoblasts (left) and myotubes (right) transduced with RAd-GFP. After image acquisition, GFP quantitation was performed in cellular lysates. Comparison between both groups was plotted as absolute B and relative-to-myoblasts fluorescenceB’. Unspecific fluorescence readings from cells alone and lysis buffer were subtracted to each group previous to data analysis. * Statistically significant difference with p < 0.05 between the experimental groups. Data values represent mean ± S.E.M. n=3 for each group. Magnification, 10X.