Figure 5: The magnetic field is a critical component of magnetofection-based gene delivery: C2C12 myoblasts and fully differentiated myotubes were incubated with either naked RAd or RAd+MagneticNanoParticles complexes and 48 hours later GFP expression was evaluated as described before. With and without magnetic field comparison was performed for both, myoblasts and myotubes. Results are plotted as absolute (A and B) and relative-to-naked virus fluorescence (A’ and B’). Unspecific fluorescence readings from cells alone, MNPs and lysis buffer were subtracted to each group previous data analysis. *Statistically significant difference with p < 0.05 between naked RAd and magnetofection with and without magnet. #Statistically significant difference with p < 0.05 between magnetofection with and without magnet. Data values represent mean ± S.E.M. n=3 for each group. fgFe/VP=femtograms of iron per Viral Particle