Figure 5: Targeting of nanozyme loaded into BMM to the sites with inflammation. Mice were intoxicated with i.p. MPTP injections (black and white bars) or i.c. 6-OHAD injections (grey and diagonal pattern bars) to produce PD model. Two (MPTP), and seven (6-OHDA) days after intoxication, BMM loaded with 3H-nanozyme (5x106/100Ál, 4 ÁCi/mouse, black and grey bars) or cell-free 3H-labellednanozyme alone (white and diagonal pattern bars) were injected intra tail vein. Four hours following nanozyme administration, mice were anesthetized, perfused, the main were collected, and the level of radioactivity was assessed. Results from N=6 mice per group (▒ SEM) demonstrated significant increase of brain BMM/nanozyme levels in mice subjected to i.c. 6-OHDA injections (grey bars) compared to mice with i.p. MPTP injections (black bars). Contrary, significantly lower nanozyme levels were recorded in kidney, spleen, liver and lungs in mice with 6-OHDA intoxications. This indicates that inflammation in peripheral organs caused by i.p. MPTP injections resulted in redistribution of the cell carriers loaded with nanozyme. No effect of peripheral inflammation was found with macrophagefree nanozyme administered without cell-carriers with the brain exception (white and diagonal pattern bars). Statistical significance between two PD models is shown by asterisk (*, p<0.05, and**, p<0.005) for BMM-incorporated nanozyme, and (#, p<0.05) for free nanozyme.