Figure 3: Tumour margin identification with SERS imaging: (A) White light image of the mouse bearing tumour, with epidermis removed. (B) SERS image of the area indicated in (A). SERS image was constructed by comparing the SERS spectra at different spatial locations (red curve in (C) and (D)) with a reference spectra (blue) collected ex-vivo. Close match of the sample and reference spectra yields an intense signal in the SERS image (white arrow) and a poor match with much lighter signal (red arrow) [19].