Total Protein (mg/ml) Naive 0.15 ± 0.01 Citrate Vehicle 1 d 0.31 ± 0.09 7 d 0.22 ± 0.02 AgNP 1 d 0.33 ± 0.02 7 d 0.18 ± 0.03 AgAc 3.88 ± 2.25

Note: Protein concentration measured by Bradford assay from recovered BAL fluid. Silver nanoparticle (AgNP) or silver acetate (AgAc) was deliverd as a 200 μg in 200 μl installation blous. Statistical significance p<0.05 compared to naïve or Citrate vehicle determined by one way ANOVA with Tukey post hoc test. n=4–5. Data displayed as mean ± SEM. Table 2: Bronchoalveolar Lavage Protein Quantification: Protein concentration measured by Bradford assay from recovered BAL Fluid following 200 μl IT instillation of citrate vehicle, 200 μg 20 nm AgNP or silver acetate (AgAc). Statistical significance p<0.05 compared to anaïve or bcitrate vehicle determined by one way ANOVA with Tukey post hoc test. n=4–5. Data reported as mean ± SEM.