Cytokine/Chemokine (pg/ml)

Citrate

 

AgNP

 

 AgAc
1 day 7 days 1 day 7 days 1 day
G-CSF 11.1 ± 3.93 53.0 ± 38.1 90.6 ± 21.1b,c 119.0±14.5 0.0 ± 0.0
GM-CSF 0.0 ± 0.0 10.9 ± 10.9 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0
MIP-1α 20.9 ± 3.7c 28.2 ± 8.9 41.5 ± 1.53b,c 40.4 ± 1.49 0.0 ± 0.0
IL-2 110.0 ±29.3c 175.0±65.3 312.0 ± 10.1b,c 328 ±23.3b 0.0 ± 0.0
IL-13 0.0 ± 0.0 20.7 ± 20.7 422.0 ± 155.0b,c 792 ± 224b 0.0 ± 0.0
IL-10 23.9 ± 8.1c 51.3 ± 31.2 101.0 ± 5.4b,c 99.8 ± 9.51 0.0 ± 0.0
INFγ 0.0 ± 0.0 187.0±187.0 126.0 ± 126.0 305.0±39.6 0.0 ± 0.0
IL-5 243 ± 43.6 351.0 ± 79.6 331.0 ± 7.8 324.0±7.82 8.74±8.74
IL-17α 54.6 ± 18.3c 94.7 ± 37.9 155.0 ± 7.8b,c 167 ± 8.32 0.0 ± 0.0
RANTES 721.0 ±26.8c 663.0±130.0 1138.0 ± 52.1b,c 924.0±60.4 143 ±13.7
Note: Serum cytokine concentrations 1 day and 7 days following 200μl instillation of Citrate vehicle or 200 μg 20 nm AgNP or silver acetate (AgAc). Statistical significance p<0.05 compared to bCitrate vehicle at matched time-point or compaired to cAgAc determined by one-way ANOVA and Tukey’s post-hoc test for multiple compairisions. Data displayed as mean ± SEM, n=4-5.
Table 3: Serum cytokines and chemokines: Serum cytokine concentrations 1 and 7 days following instillation 200 μl of citrate vehicle 200 μg 20 nm AgNP or 1 day post silver acetate (AgAc) instillation. Statistical significance p<0.05 compared to citrate vehicle b determined by student’s t-test. n=4–5. Data reported as mean ± SEM.