EC50(nM) Hillslope Maximum Stress Withdrawl (mN/mm2) Maximum Stress Constriction (mN/mm2) 5-HT Naïve 5623 ± 1304 1.67 ± 0.18   4.01 ± 0.53   Citrate 4805 ± 859 1.91 ± 0.20   2.44 ± 0.59 AgNP         1d 7769 ± 908 3.49 ± 0.50ab   3.70 ± 0.27 7d 6142 ± 980 2.65 ± 0.38   2.94 ± 0.35 AgAc 4472 ± 819 1.75 ±0.12   2.38 ± 0.52           ACh Naïve 184 ± 39 1.17 ± 0.13 4.46 ± 0.46     Citrate 190 ± 149 1.06 ± 0.20 2.30 ± 0.97   AgNP         1d 261 ± 65 0.82 ± 0.07 2.47 ± 0.49   7d 94 ± 31 1.00 ± 0.34 2.76 ± 0.49   AgAc 136 ± 199 1.08 ± 0.06 1.56 ± 0.45a             SNP Naïve 98 ± 29 1.94 ± 0.32 4.14 ± 0.58     Citrate 9 ± 3a 1.28 ± 0.09 2.40 ± 0.76   AgNP         1 d 51 ± 23 1.68 ± 0.32 3.83 ± 0.39   7d 14 ± 4a 1.49 ± 0.19 3.33 ± 0.73   AgAc 60 ± 15 1.64 ± 0.14 2.85 ± 0.86

Calculated EC50, Hillslope, Minimum Stress, and Maximum stress responses of LAD coronary artery segment to serotonin (5-HT), acetylcholine (ACh), and sodium nitroprusside (SNP) 1 day and 7 days following 200 μl instillation of Citrate vehicle or 200 μg 20 nm AgNP or 1 day post silver acetate (AgAc) instillation. Values reported as Mean ± SEM. Statistical significance p<0.05 compared to anaïve, bCitrate vehicle, 1 day AgNP determined by one way ANOVA with Tukey post hoc test. Data displayed as mean ± SEM, n=4-6. Table 4: Pharmacology of the Isolated Coronary Artery Segments: Calculated EC50, Hillslope, Minimum Stress, and Maximum stress responses of LAD coronary artery segment to serotonin (5-HT), acetylcholine (ACh), and sodium nitroprusside (SNP) 1 day and 7 days following 200 μl instillation of Citrate vehicle or 200 μg 20 nm AgNP or 1 day post silver acetate (AgAc) instillation. Values expressed ± SEM. (a) denotes significant versus Naïve (p<0.05), (b) denotes significant versus citrate vehicle (p<0.05).