S.No. Test Colony
  1   2 3 4 5   6   7 8 9 10 11                   12   Gram staining   Indole test Methyl Red (MR) test Voges-Proskauer (VP) test Citrate test Triple Sugar Iron Agar (TSI) test Mannitol Motility (MM) test Urea Hydrolysis test Nitrate Reduction test Oxidase test Sugar Fermentation test (a) Lactose (b) Adonitol (c) Dextrose (d) Trehalose (e) Melibiose (f) Raffinose (g) Arabinose (h) Sucrose (i) Cellobiose Catalase   Gram – ve rods -ve -ve -ve -ve Acid slant/ Acid Butt - / + -ve   -ve  +ve +ve   +ve +ve +ve +ve +ve -ve -ve +ve +ve +ve +ve  
  Identified thermophile   Anoxybacillus rupeinsis
Table 1: Biochemical characteristics of thermophilic bacterial isolate.