Libary (na) Richennesb ACEc Chao1d Shannone 1/Simpsonf alk (cut-off 93%)           GMR75 (63) 10 14 12 1.69 4.52 PTS01 (87) 5 9.21 6.5 0.80 1.60 ARHD (cut-off 96%)           GMR75 (88) 4 4 4 1.15 2,90 PTS01 (80) 7 13 9 1.34 3.48 bamA (cut-off 97%)           GMR75 (90) 7 20 9 1.16 2.66 PTS01 (93) 6 7 6 0.97 1.99 aNumber of gene sequences analyzed. bRichness is based on observed unique OTUs. c Nonparametric statistical prediction of total richness of different OTUs and OPFs based on distribution of abundant (10) and rare (10) OTUs. d Nonparametric statistical predictions of total richness of OTUs and OPFs based on distribution of singletons and doubletons. e Shannon diversity index. A higher number represents more diversity. f Reciprocal of Simpson’s diversity index. A higher number represents more diversity.

Table 3: Diversity indices and richness estimators of the functional gene clone libraries.