Figure 5: SDS-PAGE for molecular determination of expressed proteins from E. coli. Panel A – peh; Panels B and C – celB; Panel D – celC; Lanes 1, 2, 7, 9, semi-purified proteins; Lanes 4, 6, 8, 12, Prosieve protein marker mixture; Lanes 3, 6, 10 crude peh, celB and celC protein extract, respectively, and Lane 12, crude extract of the negative control. Bands with molecular weights of approximately 29.5, 40 and 41.5 kDa are indicated in the representative lanes of peh, celB and celC, respectively. No bands in these ranges were detected in Lane 12 confirming the expression of the tested cloned genes.