Sample RT from HPLC [M+H] + Molecular Weights (Da) Hydroxy carboxylic acid of sophorolipid Sophorolipid (standard) 9.646 648.760 650.816 647 650 C18+Na C20:1 Biosurfactant from glucose medium 9.269 675.687 691.880 708.062 675 691 707 C20+Na C20+Na Ox C18:1+Ac+Ac Biosurfactant from glucose medium 9.779 659.499 675.627 691.830 658 675 691 [C20]Lactone C20+Na C20+Na Ox Ox is the oxidized form of sophorolipids

Table 4: Retention times and [M+H] + of mass spectroscopy for peaks from standard sophorolipid, glucose medium and soybean oil medium samples.