Run Factor 1 A:temp (°C) Factor 2 B:substrate (g) Response 1 Ethanol (g/L)
1 22.00 140.00 57
2 31.00 188.28 61
3 18.27 160.00 52
4 40.00 140.00 53.5
5 31.00 160.00 74.6
6 43.73 160.00 47
7 31.00 131.72 68
8 40.00 180.00 50
9 22.00 180.00 55
Table 2: The combined effect of two factors i.e. temperature and substrate concentration in 9 combinations. Standard order 5 represents the maximum ethanol concentration.