FAME Composition Extraction – Transesterification Direct transesterification (with different solvent)
Chloroform Hexane Petroleum ether

C14:0 (% FAME)
C16:0 (% FAME)
C22:5 (% FAME)
C22:6 (% FAME)
Others (% FAME)
Total FAME content
% of algal oil (w/v)
% of biomass (w/v)
Degree of unsaturation

5.30
56.76
5.32
30.72
3.56 60.68
39.35
2.10
5.35
56.76
5.15
29.80
3.20 68.65
44.17
2.045
4.52
55.03
5.98
32.97
2.89 9.75
6.20
2.30
4.57
55.10
5.81
32.72
4.85 10.30
6.51
2.26
Table 3: FAME composition and total FAME content of the biodiesel produced from extraction-transesterification and direct transesterification methods.