| Molecular Techniques |
Target region and primers/probes used |
references |
| T-RFLP |
16S rRNA (27f-1389r) |
[99,100] |
| RFLP |
AlkB (alkH1F2/alkH3R) |
[56] |
| Dot-Blot |
nahH (nahH-F/nahH-R; C23OeF/C23OeR) nahAc (nahAc-F/nahAc-RP/nahAc-RR ;Ac114-F/Ac114-R)alkB (alkB-F/alkB-R) |
[99,100] |
| Southern-blot |
nahH (nahH-F/nahH-R; C23OeF/C23OeR) nahAc (nahAc-F/nahAc-RP/nahAc-RR ;Ac114-F/Ac114-R)alkB (alkB-F/alkB-R) |
[100] |
| RISA |
ITS (1387f/23Sr) |
[100] |
| DGGE |
16S rRNA(341F-GC/520R) |
[69] |
| DGGE |
16S rRNA(357F-GC/518R) |
[100] |
| DGGE |
16S rRNA(341-F-GC/907-R) |
[43] |
| DGGE |
16S rRNA(357F-CG/907R) |
[95] |
| DGGE |
AlkB (alkH1F2-CG/alkH3R) |
[56] |
| DGGE |
16S rRNA (907R-341F) |
[76] |
| DGGE |
Bacterial SSU rRNA (BAC27Fa/BAC518R); Archaeal SSU rRNA (Arch21f/Arch958r; Arch344fa/Arch519r) Microeukaryotic SSU rRNA (EK7F-EK516R) |
[39] |
| DGGE |
PAH-RHDαGP (PAH-RHDαGP-F ; PAH-RHDαGP-R) |
[39] |
| Clone libraries |
16S rRNA (341-F/907-R) |
[42] |
| Clone libraries |
16S rRNA (519f-1392r) |
[76] |
| Clone libraries |
alkB (alk-H1F ; alk-H3R) |
[39] |
| Clone libraries |
PAH-RHDα(PAH-RHDαGP-F/PAH-RHDαGP-R); PAH-RHDα[GN] (PAH-RHDαGN-F/PAH-RHDαGN-R) |
[55] |
| Clone libraries |
16S rRNA(27F/1492R) |
[50] |
| Clone libraries |
PAH-RHDαGP (PAH-RHDαGP-F ; PAH-RHDαGP-R); xylE (xylE-F/xylE-R) |
[69] |
| Real time PCR |
16S rRNA (341F/534R); phoA ( phoA F/phoA R) |
[50] |
| Real time PCR |
alkB (alkBFd-alkBRd) |
[77] |
| Real time PCR |
16S rRNA gene (968F-1401R) ; PAH-RHDα (GPF-GPR/GNF-GNR) |
[69] |
| Sequencing |
alkB (alkH3R) |
[56] |
| Conventional PCR |
alkB (alk-H1F/alk-H3R); PAH-RHDα(PAH-RHDαGP-F/PAH-RHDαGP-R); PAH-RHDαGN (PAH-RHDαGN-F/PAH-RHDαGN-R); bamA (BamSP9F/BamASP1R); assA/bssA (ass/bssF/ass/bssR) |
[39] |
| Conventional PCR |
PAH-RHDα (GPF/GPR; GNF/GNR); xylE (xylE-F/xylE-R) bph (bphC-F/bphC-R) |
[69] |
| Conventional PCR |
alk (L-alkB/R-alkB; L-alkB870G/R-alkB870G; L-TS2S/L-TS2Smod/L-TS2Smod2; R-deg1RE/R-deg1RE2; RH L-alkB1/RH R-alkB1; RH L-alkB2/RH R-alkB2; RH L-alkB194/RH R-alkB194; (Ac) alkM-F/(Ac) alkM-R); ndoB (L-ndoB/R-ndoB); C23DO (L-cat238/R-cat238; xylEb-F/xylEb-R; cat2,3 1a-F/cat2,3 6a-R); tod (todC1-F/todC1-R); bph (bphA1-F/bphA1-R) |
[72] |
| Competitive PCR |
cndoB (L-ndoB/R-cndoB) |
[72] |
| RT-PCR |
alkB2 (RH L-alkB2/RH R-alkB2); C23DO (cat2,3 1a-F/cat2,3 6a-R); ndoB (L-ndoB/R-ndoB) |
[72] |
| Operational Protein Families (ORFs) analysis |
PAH-RHDα(PAH-RHDαGP-F/PAH-RHDαGP-R); PAH-RHDα[GN] (PAH-RHDαGN-F/PAH-RHDαGN-R) |
[55] |
