Parameters Control BS-A BS-J BS-K BS-P
pH 7.0±0.0 8.8±0.0 8.6±0.0 8.1±0.0 8.7±0.0
Biomass yield    (c. f. u. /ml) 12±1x102 45±3x109 56±2x109 55±2x108 87±6x109
Surface tension (mN/m) 59±0.0 27±0.0 27±0.0 28±0.0 27±0.0
Emulsification index (%) - 48.1±0.36 52.0±0.30 49.0±0.20 67.0±0.45
Biosurfactant yield (g/l) 0.001±0.0002 0.875±0.0025 (25.9%)* 0.967±0.0035 (40.5%)* 0.923±0.0025 (24.6%)* 1.976±0.0000 (28.1%)*
% reduction in COD 0.0±0.00 84.2±0.05 81.6±0.12 80.4±0.30 82.5±0.20
Total sugars reduction (%) 0.0±0.00 86.4±0.02 82.3±0.03 79.4±0.15 83.5±0.03
Total nitrogen reduction (%) 0.0±0.00 70.4±0.07 63.6±0.04 59.6±0.08 68.9±0.11
Total phosphate reduction (%) 0.0±0.00 59.1±0.04 55.5±0.04 45.5±0.02 55.9±0.10
Mean + Standard deviation; n=4
*values presented in the parentheses indicates the percent rise in the yield of biosurfactant production by the respective isolates by replacing 1:3 diluted distillery waste with combination of distillery waste, curd whey and fruit processing waste in 1:1:1 ratio
Table 4: Biosurfactant production by different isolates in distillery waste combined with curd whey and fruit processing waste in 1:1:1 ratio after 120 h of incubation.