Tests Isolate BS-J Isolate BS-P
Morphological tests:
Colony morphology
Configuration Circular Circular
Margin Regular Regular
Elevation Flat Flat
Surface Smooth Smooth
Pigment Orange Light Green
Opacity Opaque Translucent
 Cell Morphology
Gram’s reaction + _
Shape Coccus Rods
Size (µm) 0.5-1 µm 2-3 µm
Arrangement Bunches Scattered
Spore (s) + _
Position central -
Shape round -
Sporangia bulging Bulged -
 Motility _ +
Physiological tests:
Growth at  temperatures
40 C + -
100 C + -
200 C + +
300 C + +
370 C + +
420 C + +
550 C - -
Growth at pH
pH  4.0 - -
pH  5.0 - -
pH  6.0 + +
pH 7.0 + +
pH  8.0 + +
pH  9.0 + +
pH 10.0 + +
pH 11.5 + +
Growth on NaCl (%)    
2.0 + +
4.0 + +
6.0 + +
8.0 + +
10.0 + +
Growth under anaerobic condition    
Biochemical tests:
Growth on MacConkey - + (NLF)
Indole test - -
Methyl red test - -
Voges Proskauer test - -
Citrate utilization - +
H2S production - +
Gas production from glucose - -
Casein hydrolysis - +
Esculin hydrolysis - +
Gelatin hydrolysis - +
Starch hydrolysis (+) -
Urea hydrolysis + +
Nitrate reduction + -
Catalase test + +
Oxidase test - +
Ornithine decarboxylase - -
Lysin decarboxylase - -
Tween 20 hydrolysis - (+)
Tween 40 hydrolysis - (+)
Tween 60 hydrolysis - -
Tween 80 hydrolysis - -
Acid production sugars
Dextrose + +
Lactose - -
Sucrose - -
+: Positive; -: Negative; (+): Weak positive; NLF: Lactose Fermenting
Table 5: Morphological, physiological and biochemical characteristics of efficient biosurfactant producing isolates.