| Tests |
Isolate BS-J |
Isolate BS-P |
| Morphological tests: |
| Colony morphology |
| Configuration |
Circular |
Circular |
| Margin |
Regular |
Regular |
| Elevation |
Flat |
Flat |
| Surface |
Smooth |
Smooth |
| Pigment |
Orange |
Light Green |
| Opacity |
Opaque |
Translucent |
| Cell Morphology |
| Gram’s reaction |
+ |
_ |
| Shape |
Coccus |
Rods |
| Size (µm) |
0.5-1 µm |
2-3 µm |
| Arrangement |
Bunches |
Scattered |
| Spore (s) |
+ |
_ |
| Position |
central |
- |
| Shape |
round |
- |
| Sporangia bulging |
Bulged |
- |
| Motility |
_ |
+ |
| Physiological tests: |
| Growth at temperatures |
| 40 C |
+ |
- |
| 100 C |
+ |
- |
| 200 C |
+ |
+ |
| 300 C |
+ |
+ |
| 370 C |
+ |
+ |
| 420 C |
+ |
+ |
| 550 C |
- |
- |
| Growth at pH |
| pH 4.0 |
- |
- |
| pH 5.0 |
- |
- |
| pH 6.0 |
+ |
+ |
| pH 7.0 |
+ |
+ |
| pH 8.0 |
+ |
+ |
| pH 9.0 |
+ |
+ |
| pH 10.0 |
+ |
+ |
| pH 11.5 |
+ |
+ |
| Growth on NaCl (%) |
|
|
| 2.0 |
+ |
+ |
| 4.0 |
+ |
+ |
| 6.0 |
+ |
+ |
| 8.0 |
+ |
+ |
| 10.0 |
+ |
+ |
| Growth under anaerobic condition |
|
|
| Biochemical tests: |
| Growth on MacConkey |
- |
+ (NLF) |
| Indole test |
- |
- |
| Methyl red test |
- |
- |
| Voges Proskauer test |
- |
- |
| Citrate utilization |
- |
+ |
| H2S production |
- |
+ |
| Gas production from glucose |
- |
- |
| Casein hydrolysis |
- |
+ |
| Esculin hydrolysis |
- |
+ |
| Gelatin hydrolysis |
- |
+ |
| Starch hydrolysis |
(+) |
- |
| Urea hydrolysis |
+ |
+ |
| Nitrate reduction |
+ |
- |
| Catalase test |
+ |
+ |
| Oxidase test |
- |
+ |
| Ornithine decarboxylase |
- |
- |
| Lysin decarboxylase |
- |
- |
| Tween 20 hydrolysis |
- |
(+) |
| Tween 40 hydrolysis |
- |
(+) |
| Tween 60 hydrolysis |
- |
- |
| Tween 80 hydrolysis |
- |
- |
| Acid production sugars |
| Dextrose |
+ |
+ |
| Lactose |
- |
- |
| Sucrose |
- |
- |
+: Positive; -: Negative; (+): Weak positive; NLF: Lactose Fermenting |
