Name of the DNA sample

Dot blot hybridization

Conventional
PCR

Real-time PCR

   

Mean of CT value

Log of DNA
concentration(ng/µL)
Conidial suspension*

+

19.75

4.2

Pure culture #

+

+

14.00

100

Assay for Artificially infected samples
Seedling    (ISD1-3)

+

22.50

0.8

Plant root  (IPR1-3)

+

21.75

1.5

Plant stem (IPS1-3) weak signal

+

19.25

6.5

Assay for Agricultural field samples
Plant root (APR1-3)

+

27.50

0.002

Plant stem (APS1-3)

+

26.75

0.021

Rhizosphere soil (ARS1-3)

+

33.25

0.0015

*-5×106 conidia mL-1, + presence, − absence, #- F. udum 193652
Table 3: Evaluation of species specific probe and primers under three different assays.