Figure 3: qPCR differentiation with mixed X. fastidiosa subspecies. Fluorescence data acquired using molecular beacon® R383MB, specific for X. fastidiosa subsp. multiplex, with primers RMB307F and RMB536R under described reaction conditions. Data shows differentiation X. fastidiosa subsp. multiplex and fastidiosa from mixed DNA samples. Reaction samples are A) X. fastidiosa subsp. multiplex, B) 50% X. fastidiosa subsp. multiplex, C) 20% X. fastidiosa subsp. multiplex, D) 100% X. fastidiosa subsp. fastidiosa.