Primer Sequence TbD133F CAAGGAAAACCCATCGAAGT TbD286R TGAACGTGACTGGTACTTGGA TbD287R TTGAACGTGACTGGTACTTGG TbD290R GGGTTGAACGTGACTGGTACT TbD291R CGGGTTGAACGTGACTGGTA ZgbrTm01F ATGGACAACACAGCAAGCTC ZgbrTm02F TGTATGGACAACACAGCAAGC ZriTm01F CGGTTTGGATTTTGTTTGGA ZriTm02F ATCGCGGTTTGGATTTTGTT ZTm306F CGTCAGACTACGCCAGATCA ZTm489F TTAAGCGTCGTTTTGGTACG ZTm498F GTTTTGGTACGCGCTTTCTT ZTm503F GGTACGCGCTTTCTTGATGT ZTm579-20R GACGACCAATTAAACCGTGA ZTm579-21R GACGACCAATTAAACCGTGAA ZTm597R GTACGCACACTATGGCGAGA ZTm608R GCTTAGGAAGGGTACGCACA *Primers beginning with T are from the tonB region and primers beginning with Z are from the zot region

Table 1: Primers designed to amplify regions of the tonB and zot gene regions for Tm analysis of X. fastidiosa subspecies*.