Fluorescence Method X. fastidiosa subsp. Average Tm Error °C   fastidiosa 85.42° C 0.3869 SYBR Green® multiplex 85.66° C 0.0520   sandyi 85.89° C 0.0346   fastidiosa 86.58° C 0.0458 Eva Green® multiplex 86.43° C 0.0346   sandyi 86.87° C 0.0458   fastidiosa 84.90° C 0.0346 Takara SYBR Green® multiplex 85.30° C 0.0346   sandyi 85.57° C 0.0346

Table 2: Average Tm & error for differentiation of X. fastidiosa subspecies using three methods of fluorescence under ideal melt conditions with primers in Table 1.