| Primer |
Type |
Position on gene |
Length |
Sequence(5′-3′) |
| F |
Forward |
701-720 |
20 mer |
GTCTTATGAGCAACGGGATG |
| B |
Backward |
867-887 |
21 mer |
GAACATGACCTGATTAGTGTG |
| F3 |
Forward outer |
387-405 |
19 mer |
TGCAGTCCGTTGAGGAAAC |
| B3 |
Backward outer |
598-617 |
19 mer |
CCTGTACGTCCATGATCGTC |
| FIP |
Forward inner |
453-473 and 413-431 |
40 mer |
AGTCACGGGCCCTTACAACAGCCCAATACATTGGGCCACG |
| BIP |
Backward inner |
515-534 and 564-581 |
37 mer |
TCGAAGGTTCGCCGAAGGCGACAATGGGGACAGCAGC |
|
