Figure 5: Optimization of RT-LAMP reaction for detecting of PLRV: a) Effects of primer concentrations; 5 pmol concentrations for F3 and B3 primers, and 20 pmol for LF, LB, FIP and BIP were selected as optimized concentration of primers; b) Effects of Betaine and MgSO4 concentrations; 100 mM concentration for Betaine and 1mM concentration for MgSO4 were selected as optimized concentrations. Lanes: M, 100 bp ladder.