Primer Type Position on gene Length of primer Length of product Sequence(5'-3') Forward Forward outer 85-104 20 nt 336 bp CGCGCTAACAGAGTTCAGCC Backward Backward outer 420-401 20 nt   GCAATGGGGGTCCAACTCAT F3 Forward outer 61-78 18 nt   AGAAGGCAATCCCTTCGC B3 Backward outer 245-264 20 nt   GGTGAAACTTCCTTGGGTGT FIP Forward inner 139-159 and 79-96 43 nt 163 bp GCCTCCTCTTCTGCGTCTTCGTTTTAGGCGCGCTAACAGAGTT BIP Backward inner 161-182 and 223-241 45 nt   ATCGCCGCTCAAGAAGAACTGGTTTTCCACGAGGTTGTCCTTTGT LF Loop forward outer 97-118 22 nt   CCGTGACCATAACCACTGGCTG LB Loop backward outer 191-210 20 nt   GAGGACGAGGCTCAAGCGAG

Table 1: Oligonucleotide primers used for RT-LAMP and RT-PCR of coat protein gene of PLRV. RT-PCR primers (forward and backward) were designed by Oligo7 software that amplifies a 336 bp fragment. RT-LAMP primers (F3, B3, FIP, BIP, LF and LB) were designed by PrimerExplorer V.3 that produced many fragments with different sizes from which the smallest fragment was 163 bp in size.