Figure 2: A: PCR amplification for cloning of the P19 Egh gene. Lane M: Molecular weight standard 1 Kb plus DNA ladder (Sib Enzyme); Lane 1: circular pCR 2.1-TOPO plasmid vector; Lane 2: P19 Egh recombinant plasmid; Lane 3: amplified P19 Egh gene by specific P19 primer from recombinant plasmid, and Lane 4: amplified P19 Egh gene from recombinant plasmid by M13 primer. B: SDS-PAGE for the recombinant p19 fractions and purifications. C: Western blot for the purified P19 using the TBSV antiserum.