| |
Primer |
Primer sequence 3̀→5̀ |
Annealing °C |
| Real time PCR |
Chitinase1 Forward |
TTC GTG GAG TGC ATC GGT |
60 |
| Chitinase1 Reverse |
TAN GCN CNN NCC NGG RTT |
| Defensin 1 Forward |
CAA TGT AAC TTA AAG TGC CTA ATT ATG |
55 |
| Defensin 1 Reverse |
CTT ATC AGA TCT CAA TGG AGA AAT C |
| Defnsin 2 Forward |
TCA CCA AAC TAT TGG ATT TCA A |
57 |
| Defensin 2 Reverse |
GAC TCA ATT TTT GAC TTC TTA ATCC |
| Differential PCR |
EZ |
GCATCACAGACCTGTTATTGCCTC |
45 |
| Chi15 |
GGYGGYTGGAATGARGG |
55 |
| EzA1A13 |
CAG GCC CTT CCA GCA CCCAC |
53 |
| Chi 25 |
GAYTTRTGGGAATAY CC |
52 |
| PR1 |
CCCCAAGCTTTTAGTATGGCTTCTCGTTCACATAAT |
40 |
| PR2 |
CGCGGATCCTACTGGCTATTCTCGATTTTTAATCG |
40 |