| Primer |
Type |
Position on gene |
Length |
Sequence(5’-3’) |
| F |
Forward |
8721–8740 |
20mer |
ACGTCCAAAATGAGAATGCC |
| B |
Backward |
9181–9200 |
20mer |
TGGTGTTCGTGATGTGACCT |
| F3 |
Forward outer |
8870–8890 |
21mer |
ATACGACATAGGAGAAACTGA |
| B3 |
Backward outer |
9059-9078 |
20mer |
ACGCTTCTGCAACATCTGAG |
| FIP |
Forward inner |
8931–8951 and 8900–8920 |
42mer |
GTTTGGCGAGGTTCCATTTTC-TGTGATGAATGGGCTTATGGT |
| BIP |
Backward inner |
9004–9024 and 9036–9056 |
42mer |
TGAAACCAATCGTTGAGAATG-ATGTGCCATGATTTGCCTAAG |
|
