Assay Total time Detection method Safety Need to UV ray Need to detect instruments Cost User Friendly Accuracy Need to RNA extraction Need to cDNA synthesis separately DAS-ELISA 1-2 days Visual + ELISA reader Yes No Yes High Low High No No RT-PCR 5 hours Gel Electrophoresis No Yes Yes High Low High Yes Yes RT-LAMP 120 minute Visual+ Gel Electrophoresis Yes No and  Yes No and  Yes Low High High Yes No IC-RT-PCR 3 hours Gel electrophoresis No Yes Yes High High High No Yes IC-RT-LAMP 90 minute Visual+ Gel electrophoresis Yes No and  Yes No and  Yes Low High High No No Colorimeric IC-RT-LAMP 90 minute Visual Yes No No Low Very high High No No

Table 2: Comparison of DAS-ELISA, RT-PCR, RT-LAMP, IC-RT-PCR and IC-RT-LAMP assays.