Table 3

Treatments	Healthy control	Viral control	Decoction of black seed	Infusion of black seed
*In vitro*
No. of leaves	7.01 ± 0.74^b	6.20 ± 0.63^b	8.60 ± 1.70^a	8.2±1.40^a
Shoot length (cm)	30.00 ± 3.09^b	29.6 ± 3.69^b	42.6± 8.37^a	39.6 ± 5.52^a
Fresh weight (g)	10.13± 2.51^b	7.92 ± 1.95^b	11.63 ± 2.02^a	12.53 ± 2.60^a
Pre inoculation treatment
No. of leaves	5.5 ± 0.53^bc	5.00 ± 0.82^c	8.7± 0.48^a	6.00 ± 0.82^b
Shoot length (cm)	35.00 ± 4. 11^a	26.6 ± 3.06^b	35.00 ± 0.84^a	30.00 ± 5.23^b
Fresh weight (g)	10.77 ± 1.81^a	5.86 ± 1.71^c	12.00 ± 5.23^a	11.92 ± 1.22^a
post inoculation treatment
No. of leaves	5.5 ± 0.53^bc	5.00 ± 0.82^c	7.40 ± 0.52^a	5.70 ± 0.48^b
Shoot length (cm)	35.00 ± 4. 11^a	26.6 ± 3.06^c	31.40 ± 3.37^b	32.8 ± 4.24^ab
Fresh weight (g)	10.77 ± 1.81^a	5.86 ± 1.71^d	8.72 ± 0.58^b	7.20 ± 0.98^c

Healthy control=twenty plants normal (without any treatments) all are healthy. Viral control=squash seedling mechanically inoculated with ZYMV on cotyledonary leaves and first stage leaves.
^a,b,c,d means in the same row with different superscript differ significantly (p<0.05)

Table 3: Effect of black seed extraction in vitro and in vivo on growth parameters of mechanically infected squash plants by ZYMV. (Each value is the mean twenty reading ± SD).