Table 1

Reaction	Number of cycles	Thermal settings
Primary	1	93°C (1min); 95°C (5min)
	5	94°C (30sec); 62°C (1min); 72°C (2min30sec)
	1	94°C (30sec); 25°C ramping 72°C (3min); 72°C (2min30sec)
	15	94°C (20sec); 65°C (3min30sec);
		94°C (20sec); 65°C (3min30sec);
		94°C (30sec); 42°C (1min); 72°C (2min30sec)
	1	72°C (5min); 4°C Hold
Secondary	12	94°C (20sec); 65°C (3min30sec);
		94°C (20sec); 65°C (3min30sec);
		94°C (30sec); 42°C (1min); 72°C (2min30sec)
	1	72°C (5min); 4°C Hold
Tertiary	30	94°C (30sec); 42°C (1min); 72°C (2min30sec)
	1	72°C (5min); 4°C Hold

Table 1: HE-TAIL PCR cycle settings. Primary, secondary and tertiary nested PCR reactions were performed sequentially. The primary PCR reaction consists of 15 TAIL cycles, while the secondary reaction contains 12 TAIL cycles.