| Reaction |
Primer |
Type |
Length of Primer |
Sequence(5`-3`) |
Length of Product |
| PCR |
F |
Forward |
24 nt |
TGCTCCAATAAGGTGCTTCCAGTG |
292 bp |
| PCR |
B |
Backward |
25 nt |
TTTCCTCTGTCCTCATTCACAAACG |
| LAMP |
F3 |
Forward outer |
18 nt |
AAGTAAGTGGGATCTACG |
Fragment with different sizes |
| LAMP |
B3 |
Backward outer |
21 nt |
GAAATGCAAGAATGGGCTGAT |
| LAMP |
FIB |
Forward inner |
47 nt |
GGCCCACAATTACATCACAGGGCTTTTGACGTTGTATTCCACTTCAT |
| LAMP |
BIP |
Backward inner |
53 nt |
CCACATAGTCTTCCCTGTTCTTGATTTTTATTTTGGGGTTGATGCCGCTGCGC |
| LAMP |
LF |
Loop forward |
23 nt |
TAGATTTTAGCCTTAGAACATAT |
| LAMP |
LB |
Loop backward |
21 nt |
ACTATGATACTATTATATCTT |
|
