| Visual system |
Detection method |
Safety |
Positive reaction |
Negative sample |
Need to UV ray |
Stability |
Cross contamination |
Use time |
| magnesium Sulfate |
Visual |
Yes |
Turbidity |
No turbidity |
No |
5-10 seconds |
No |
Before the amplification |
| Calcium chloride |
Visual |
Yes |
Turbidity |
No turbidity |
No |
40-60 seconds |
No |
Before the amplification |
| SYBR® Premix Ex TaqTM II |
Visual |
Yes |
Green |
Red |
Yes |
1–3 days |
Yes |
After the amplification |
| Hydroxynaphthol blue |
Visua |
Yes |
Sky blue |
Violet |
No |
2-3 weeks |
No |
Before the amplification |
| GeneFinderTM |
Visual |
Yes |
Green |
Red |
No |
2-3 weeks |
No |
Before the amplification |
| SYBR Green I |
Visual |
Yes |
Green |
Red |
Yes |
2-3 weeks |
No |
Before the amplification |
| Ethidium bromide |
Visual |
No |
Yellow |
No color |
Yes |
2 weeks |
Yes |
After the amplification |
|
