Strain Name Locus Definition Primer used Sequence bp T. viride 01PP JX119211 Hypocrea rufa isolate 01PP-8315/11 18S ribosomal RNA gene, partial sequence; Internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28 S ribosomal RNA gene, partial sequence. ITS-1tccgtaggtgaacctgcgg ITS-2 tcctccgcttattgatatgc 1173bp T. harzianum Th azad KC800922 Trichoderma harzianum isolate Th-azad/CSAU 6796 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 4, partial sequence. ITS-1agagtttgatcctggctcag ITS-4ggttaccttgttacgactt 546bp T. asperellum Tasp/CSAU KC800921 Trichoderma asperellum isolate Tasp(CSAU)-8940 18s ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8s ribosomal RNA gene, and internal transcribed spacer 4, partial sequence ITS-1tccgtaggtgaacctgcgg ITS-4 tcctccgcttattgatatgc 641 bp T. koningii Tk (CSAU) KC800924 Trichoderma koningii isolate Tk (CSAU) 5201 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 4, partial sequence. ITS-1 tctgtaggtgaacctgcgg ITS-4 ggaagtaaaagtcgtaacaagg 206 bp T. atroviride 71L KC 008065 Trichoderma atroviridestrain TAU8 18S ribosomal RNA, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA, and internal transcribed spacer 2 complete sequence; and 28S ribosomal RNA partial sequence. ITS-1tcctccgcttattgatatgc ITS-2 ggaagtaaaagtcgtaacaagg 627bp T. longibrachiatum 21PP JX978542 Trichoderma longibrachiatum strain 21PP 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence ITS-1tcctccgcttattgatatgc ITS-2 ggaagtaaaagtcgtaacaagg 664 bp T. virens Tvi (CSAU) KC800923 Trichoderma virens isolate Tvi (CSAU)-417718S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 4, partial sequence. ITS-1 tcctccgcttattgatatgc ITS-4 ggaagtaaaagtcgtaacaagg 635 bp

Table 4: Submission of the gene sequences at NCBI Database.