Sr. Primer seq 5’- 3’ Phase %GC Tm
1  F
R		 GGACTATCTAGTTTATGGAGGAG
AGGCAAAACGAGCAGTGTAA		 Dominant-coupling 43.0
45.0		 56.6
62.3
2  F
R		 CTTGTATTGTGCGTTGGAA
CTTGTATTGTGCGTTGGAA		 Dominant-repulsion 45.0
42.0		 59.2
59.7
3   F
R		 CGAGGGCTACATTCAAGAGC
TCCAAGGGTCTTCTCGGTAA		 Designed by primer 3+
(NCBI)		 55.0
50.0		 60.0
59.7
Table 2: Details of rust resistance linked primers used in the study of oat genotypes.