Name Sequence (5’ à 3’)a Applications
Cut1F CTTGTCGCTGCCGCTCCTGT Reverse transcription PCR
Cut1R AGAGTCGCTTAGCCTCGTTGAT Reverse transcription PCR
gCUT-F AATCCCTTACAACTTTCCTCTGACA Cutinase gene amplification
gCUT-R ATCAACCGCCACGATACAGACAA Cutinase gene amplification
SmaI-CutS-F TATATAcccgggTCATCTCTCTTGCCGTTTC Cassette construction, Southern analysis
XbaI-CutS-R TCAGATtctagaGTCCAAGGTTCTGCAGGTTC Cassette construction, Southern analysis
BamHI-CutAS-F TAGTATggatccGTCCAAGGTTCTGCAGGTTC Cassette construction
XhoI-CutAS-R ATTCGActcgagTCATCTCTCTTGCCGTTTC Cassette construction
S-GFP-R TCGCCGATGGGGGTGTTCTGCT PCR, Southern analysis
5NQ.CUTF TCTGTTCGGCTACACCAAGAA Quantitative real-time PCR
5NQ.CUTR CAAGAAATGCGCCGGCAGGAT Quantitative real-time PCR
hphF GCTGCGCCGATGGTTTCTACA pAA1 analysis
hphR GCGCGTCTGCTGCTCCAT pAA1 analysis
aRestriction sites are bolded lower case letters
Table 1: List of primers used in the study.