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Figure 2: Electrophoretic mobility of P42 (CP), A; P33, B and P22, C genes of TBSV Egh purified PCR product amplified from cDNA using specific synthetic oligonucleotides primers. Approximately 5 μl of P42 gene of TBSV Egh isolate was run through 1% agarose gel in 1xTBE buffer and stained with ethidium bromide and photographed. A: Lane M: Fermentase Low Molecular Weight Standard (100bp to 1000bp); lane2: P42 of TBSV Egh. B: Lane M: Fermentase Molecular weight standard (100bp-1Kbp) DNA ladder Lane1: P33 gene of TBSV Egh. C: Lane M: Molecular Weight Standard 1Kb plus DNA ladder (Sib Enzyme); Lane1: P22 gene of TBSV Egh cDNAs. |