Figure 4: Electrophoretic mobility of plasmid DNA containing sequences of CP ER gene of TBSV Egh isolate from E.coli strain TOP10 F' transformants on 1% agarose gel stained with ethidium bromide. LaneM: Molecular weight standard 1 Kb plus DNA ladder (Sib Enzyme); Lane1: circular pCR 2.1- TOPO plasmid vector; Lane2: CP ER recombinant plasmid; Lane3: PCR product of CP ER gene.