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Figure 4: The Neighbour-joining tree of W. circinata isolates based on ITS sequence analysis. Bootstrap values 65% and above assessed by 500 replications are shown next to the branches. The tree is midpoint rooted. The clades of W. circinata varieties circinata, oryzae, zeae and unidentified W. circinata group (UWC) are indicated on the tree. Taxons starting with AB, AJ, GU, HM, and HQ are GenBank accessions. UWC isolate BSF 13 grouped closed to W. circinata variety prodigus (accessions HM597147, HQ850254, and HM597146) whereas UWC isolate ANP 109B is closely clustering with variety agrostis (AB213578 and AB213572). GU045342 and AB213594 are W. circinata var. zeae ITS sequences. AB213588, AJ000195 and AB213589 are W. circinata var. oryzae ITS sequences while AB213581 and AB213582 belong to W. circinata var. circinata. |