Substance Calibration regression equation Retention times (min)
Lactic acid C=2.36 × 10-7 × p 12.7
Acetic acid C=4.08 × 10-7 × p 15.7
Formic acid C=2 × 10-6 × p+0.0576 14.4
Propionic acid C=2 × 10-6 × p+0.0218 18.5
Butyric acid C=2 × 10-6 × p+0.0182 16.7
Succinic acid C=1.97 × 10-7 × p 12.2
Acetaldehyde C=4.27 × 10-7 × p 17.5
Ethanol C=3.85 × 10-7 × p 22.6
C: The concentration of the analyzed substances (mg/100 ml).
P: Peak area.
Table 1: Regression equation and retention time for HPLC standards.