Loci Segregants Marker Fragment Lr24 gene & marker fragment Linkage ++/R -+/R --/R ++/H -+/H --/H ++/S -+/S --/S χ21:2:1 P χ21:2:1:2:4:2:1:2:1 P χ2 P Lr24-Xgwm11 41 0 0 4 89 2 0 0 46 0.1866 0.9109 326.87 0.00 329.68 0.00 Lr24-Xbarc71 41 0 0 2 93 0 2 0 44 0.0987 0.9518 326.69 0.00 336.70 0.00

R: Homozygous Resistant; H: Heterozygous; S: Homozygous Susceptible Marker Fragment ++: Homozygous Resistant +-: Heterozygous --: Homozygous Susceptible

Table 2: Test of linkage between the leaf rust resistance gene Lr24 and SSR markers (Xgwm114 and Xbarc71) in the F2 population of the cross PBW343+Lr24 X CSP44- Lr48 of wheat.