Per one reaction Final concentration Basal concentration Material 15.2μl - - Steriledouble distilled water 2.5μl 1X 10X PCR buffer 1.6μl 3.2Mm 50Mm MgCl2 1.5μl 0.6Mm 10Mm dNTPS 0.2 μl 1Unit 5Unit/μl Taq DNA polymerase enzyme 21 μl - - *Total volume of master mix

Table 2: Ingredients and amounts for the preparation of master mix used in the PCR reaction. *Final volume of PCR reaction: (2 μl DNA+2 μl Primer+21 μl master mix)=25 μl.