Number of cycles Conducted Steps Time Temperature (°C) 1Cycle Initial denaturation 5min 94 35 Cycles Denaturation 1 min 94 Annealing 75 second Depending on the type of primer used Extension 2 min 72 1 cycle Final extension 10 min 72

Table 3: Time and temperature required for conducting different stages of PCR for primers gpd1, gpd2 and specific UBC primers.