Fraction
Percentage of solvent
Volume of solvent used
Fraction 1
100%CHCl
3
50 ml
Fraction 2
25%EtOAC: 75%CHCl
3
50ml
Fraction 3
50%EtOAC: 50%CHCl
3
50ml
Fraction 3
50%EtOAC: 50%CHCl
3
50ml
Fraction 4
100%EtOAC
50ml
Fraction 5
50%EtOAC: 50%MeOH
50ml
Fraction 6
100%MeOH
50 ml
Table 1:
Organic solvents used as eluting solvent in column chromatography.
Note: CHCl
3
: chloroform; EtOAC: Ethyl acetate; MeOH: Methanol.
