Solvent system Concentration of resin mg/100 ml MEAP
6 5 4 3 2.5 1.5 Control
Chloroform (CHCl3) GD (mm) 12.30 ± 0.01g 13.80 ± 0.01f 16.30 ± 0.02e 18.60 ± 0.03d 20.80 ± 0.06c 26.30 ± 0.04b 30.60 ± 0.01a
GR (mm) 0.9 ± 0.1c 1 ± 0.5bc 1.2 ± 0.1bc 1.2 ± 0.2bc 1.3 ± 0.1b 1.9 ± 0.1a 2.25 ± 0.03a
%INH 60.49 ± 0.02a 50.17 ± 0.03b 46.16 ± 0.4c 37.32 ± 0.03d 29.35 ± 0.1e 13.18 ± 0.03f 0.00
Ethanol (EtOH) GD (mm) 9.40 ± 0.02e 12.40 ± 0.01de 15.40 ± 0.01cd 17.20 ± 0.02c 18.60 ± 0.02c 22.00 ± 0.2b 30.60 ± 0.01a
GR (mm) 0.6 ± 0.1d 0.9 ± 0.1cd 1 ± 0.6c 1.08 ± 0.02c 1.14 ± 0.03c 1.56 ± 0.03b 2.25 ± 0.03a
%INH 66.19 ± 0.02a 57.08 ± 0.02b 49.04 ± 0.26c 40.28 ± 0.48d 33.68 ± 0.03e 23.82 ± 0.02f 0.00
Ethyl acetate (EtOAC) GD (mm) - 11.30 ± 0.01f 13.10 ± 0.02e 16.90 ± 0.01d 19.20 ± 0.03c 22.90 ± 0.01b 30.60 ± 0.01a
GR (mm) - 0.76 ± 0.02e 0.77 ± 0.03e 1.14 ± 0.02d 1.3 ± 0.02c 1.55 ± 0.03b 2.25 ± 0.03a
Methanol (MeOH) GD (mm) - - 5.00 ± 0.1e 9.00 ± 0.1d 11.60 ± 0.02c 13.30 ± 0.03b 30.60 ± 0.01a
GR (mm) - - 0.10 ± 0.05d 0.57 ± 0.01c 0.65 ± 0.03b 0.68 ± 0.02b 2.25 ± 0.03a
%INH 100a 100a 79.85 ± 0.02b 67.76 ± 0.03c 58.36 ± 0.03d 51.06 ± 0.00e 0.00
Table 3: In vitro inhibitory effect of resin from J. procera in the growth diameter, growth rate and percentage mycelial growth inhibition of P. demidoffii, dissolved by different solvent after 20 days of incubation at 25°C.
Note: Results are the mean values ± standard deviations. Means within the column under a parameter having a common letter do not differ significantly at (p<0.05). %INH: percentage of mycelial inhibition. GD: Growth Diameter GR: Growth Rate -: No growth (100% inhibition).