| Fraction |
Extraction solvent |
Developing solvents |
| Benzene:Methanol (18:6) |
Benzene:Ethanol:Ammonia (18:2:1) |
| Fraction 1 |
100%CHCl3 |
0.68, 0.78 |
0.60, 0.72 |
| Fraction 2 |
25% EtOAC:75%CHCl3 |
0.50, 0.56 |
0.64 |
| Fraction 3 |
50% EtOAC:50%CHCl3 |
0.50, 0.55, 0.62 |
0.48, 0.60 |
| Fraction 4 |
100%EtOAC |
0.46, 0.62 , 0.67 |
0.45, 0.56 |
| Fraction 5 |
50% EtOAC:50%MeOH |
0.41, 0.52 |
0.50 |
| Fraction 6 |
100%MeOH |
0.18, 0.25 |
0.42.0.38 |
Note: CHCl3: Chloroform EtOAC: Ethyl acetate MeOH: Methanol. |
