Figure 2: Transmission electron micrographs of partially purified preparation of naturally infected gladiolus showing flexuous rod shaped virus particles of size 720 × 11 nm (a). Ultrathin sections of infected gladiolus leaves observed under TEM showing laminated inclusions (b) and scrolls (c). Virus detection by western immuno blot assay (d) using BYMV antiserum showing positive bands of ~35 kDa in naturally infected gladiolus, experimentally inoculated gladiolus and V. faba (lanes 2, 3 & 4) similar to BYMV infected P. peruviana taken as a positive control (lane P), PageRuler™ Prestained Protein Ladder as protein marker (Lane M). Virus detection by RT-PCR (e) 1% Agarose gel electrophoresis of RT-PCR products of potyvirus infected gladiolus samples (1-5) using potyvirus degenerate primers Pot I/II. M = λ-DNA EcoRI/HindIII as DNA marker.