Components Volume per reaction 10 x RT buffer 2 μ dNTP mix (10 mM) 2 μl Random Primers (10 mM) 2 μl RNAase inhibitor (1 U/μl) 1 μl Reverse transcriptase (5 U/μl) 1μl RNA (1 μg) x μl H2Odest 0 - x μl Total volume 20μl

Table 2: Pipetting scheme containing reverse transcription reagents.