Components
Volume per reaction
Fast SYBR
®
- Green Master Mix
10 μl
Forward primer (10 mM)
0,5 μl
Reverse primer (10 mM)
0,5 μl
DNA (1:10 diluted)
1 μl
H
2
Odest
8 μl
Total Volume
20 μl
Table 4:
Pipetting scheme containing Real time PCR Fast SYBR
®
- Green Master Mix.
