| Locus |
Sequences of Primer
5’ ------------------------ 3’ |
Base pair(bp) |
Annealing temperature and duration |
Reference |
| 16srDNA |
Xcc16S-F:5’-GCAAGCGTTACTCGGAATTA-3’
Xcc16S-R: 5’-TACGACTTCACCCCAGTCAT-3’ |
959 |
56°C for 30 Sec |
Used in this study |
| efP gene |
DXEP1F: 5’-TCATCACCGAGACCGAATA-3’
DXEP1R: 5’-TCCTGGTTGACGAACAGC-3’ |
434 |
54°C for 30 Sec |
Used in this study |
| hrp gene |
DhrpXccF:5’-GTGGCCATGTCGTCGACTC-3’ DhrpXccR:5’-GGAATAAACTGTTTCCCCAATG-3’ |
769 |
60°C for 40 Sec |
Singh et al., 2014 |
| BOX |
BOXA1R: 5’-CTACGGCAAGGCGACGCTGACG-3’ |
Multiple bands |
53°C for 1min |
Schaadet al. 2001 |
| ERIC |
ERIC1R: 5’-ATGTAAGCTCCTGGGGATTCAC-3’
ERIC2: 5’-AAGTAAGTGACTGGGGTGAGCG-3’ |
Multiple bands |
46°C for 1min |
Schaadet al. 2001 |
| REP |
REP1R: 5’-IIIICGICGICATCIGGC-3’
REP21: 5’- ICGICTTATTATCIGGCCAC-3’ |
Multiple bands |
48°C for 1 min |
Schaadet al. 2001 |
